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Recent advances in mass spectrometric instrumentation enable the identification and quantification of peptides and phosphopeptides at an unprecedented depth. Despite the ever increasing sequencing speed of modern mass spectrometers, single measurements are often not sufficient to fully resolve the (phospho)proteome complexity and thus coverage must generally be improved by some form of two-dimensional peptide fractionation. In shotgun proteomics, the most widely applied second dimension of peptide separation is reversed-phase C18 chromatography directly coupled to tandem mass spectrometry. The Preciex® Fractionation Stage-tips provide several orthogonal first dimension separations, i.e. SCX, Basic RP, and Hilic, which allow for enhancing specific proteome sample deep coverage by online and/or offline mode. Process of use: centrifugation, vacuum, positive pressure.

Formats: 10µL, 200µL, 350µL and 1mL.

For any other format or online columns, contact us.  *200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.  

Features of Fractionation Stage-tip:

• Easy-to-use
• Higher recovery of low amount sample
• Various chromatographic mechanisms for theꦅ best solution

• Suitable for manual and automated processing

Common product Specifications:

Sample	peptide	peptide	peptide
Chromatographic bed	SCX, 120Å	BRP18, 120Å	HILIC, 120Å
Volume	200μL	200μL	200μL
Capacity	10ug	10ug	10ug
PH Range	2-8	1-12	2-8
Temperature	4-60°C	4-60°C	4-60°C
Quantity	96 Tips	96 Tips	96 Tips
Detection Method	HPLC, Mass spectrometry, Capillary electrophoresis, Atomi෴c absor🅰ption spectroscopy

Ordering Information:

Description	Format	Capacity	Part Number
Preciex Fractionation, SCX	10μL	2μg	2103021
Preciex Fractionation, SCX	200μL	10μg	2103102
Preciex Fractionation, SCX	200μL	30μg	2103133
Preciex Fractionation, SCX	350μL	100μg	2103104
Preciex Fractionation, SCX	1000μL	500μg	2103505
Preciex Fractionation, BRP	10μL	2μg	2104021
Preciex Fractionation, BRP	200μL	10μg	2104102
Preciex Fractionation, BRP	200μL	30μg	2104133
Preciex Fractionation, BRP	350μL	100μg	2104104
Preciex Fractionation, BRP	1000μL	500μg	2104505
Preciex Fractionation, Hilic	10μL	2μg	2105021
Preciex Fractionation, Hilic	200μL	10μg	2105102
Preciex Fractionation, Hilic	200μL	30μg	2105133
Preciex Fractionation, Hilic	350μL	100μg	2105104
Preciex Fractionation, Hilic	1000μL	500μg	2105505

 

User Guide

Material Safety Data Sheets (MSDS)